PCR testing of urogenital infections

PCR

The RealBest technology covers all necessary steps of real-time PCR analysis: from reagents for specimen transportation and nucleic acid extraction to software for results processing

Баннер_1.png


Specimen collection and nucleic acid extraction

Most of the urogenital diseases share common clinical specimens for direct identification of pathogens by the PCR method

For women
  • Urethral, cervical and vaginal swabs, rectal swabs
  • The first portion of the urine
  • In rare cases, for specific reasons:
    oropharyngeal swabs, paraurethral
    and greater vestibular glands secretion,
    ocular secretions

4.png

For men
  • Urethral swabs
  • The first portion of the urine
  • In rare cases, for specific reasons:
    rectal swab, oropharyngeal swab,
    prostate gland discharge, ocular secretions
    •   

Cat. №

Kit Name

Number of tests

1.png

Epithelial cells swabs should be collected in tubes with transport solution:

8894

Transport solution (1) – 0,3 mL

100

8885

Transport solution (2) – 1 mL

200

2.png

Manual DNA extraction:

8899

RealBest DNA-express (the kit already includes tubes with transport solution)
Only 15 min protocol!

100

8889

RealBest DNA-extraction 3 (variant 6×16)

96 (6x16)

8896

RealBest extraction 100*  

48 (8x6)

 3.png Automated DNA extraction:

8878

RealBest DNA-extraction 3 (variant 2x48)

96 (2x48)

8883

RealBest UniMag Universal NA Extraction Kit*

96 (4x24)

*Kits are suitable for DNA extraction from cervical swabs in liquid-based cytology medium


Features of RealBest PCR kits for amplification

Баннер_21.png

  • Lyophilized ready Master Mix in 0.2 ml tubes – only requires addition of extracted DNA/RNA sample
  • Universal amplification protocol
  • Reliability of analysis: kits contain Noncompetitive Internal Control sample
  • Convenience of storage and transportation: no freezing required, transportation up to 26 °C for no more than 10 days
  • 96-tube plate compatible with plate-like thermal cyclers

Compatible thermal cyclers: CFX96 (Bio-Rad, USA), DTprime (DT-96), DTlite (DNA-Technology, Russia), Gentier 96E/R (Xi’an TianLong, Science and Technology Co., Ltd., China), RBfast (Vector-Best, Russia) and others with similar characteristics after validation.

5.png

8888 RealBest Sample Validation

The kit for validation of sample quality by quantitative assessment of human DNA content in clinical specimens (swabs). Can be used for quantitative determination of the pathogen content in the sample.

Customized combination of analyzed pathogens can be assembled from different RealBest kits and processed in one run due to universal amplification protocol. For instance:

Баннер_5.png


Sexually Transmitted Infections

Cat. №

Singleplex and duplex format

1998

RealBest DNA Chlamydia trachomatis

2098

RealBest DNA Trichomonas vaginalis

4396

RealBest DNA Mycoplasma genitalium

4498

RealBest DNA Neisseria gonorrhoeae

4494

RealBest DNA Neisseria gonorrhoeae test 2

1898

RealBest DNA Treponema pallidum


0455

RealBest DNA C. trachomatis/Ureaplasma species

0492

RealBest DNA C. trachomatis/Ureaplasma urealyticum

0490

RealBest DNA C. trachomatis/M. genitalium

0498

RealBest DNA C. trachomatis/T. vaginalis

0457

RealBest DNA C. trachomatis/N. gonorrhoeae

0494

RealBest DNA M. hominis/M. genitalium

0496

RealBest DNA T. vaginalis/N. gonorrhoeae

0477

RealBest DNA T. vaginalis/Gardnerella vaginalis
   
Cat. №  Complex analysis for STIs

0488

RealBest PCR-12 STI
Differential detection of: Chlamydia trachomatis, Ureaplasma spp., Mycoplasma hominis, Mycoplasma genitalium, Trichomonas vaginalis, Neisseria gonorrhoeae, Candida albicans, Gardnerella vaginalis, CMV,
HSV 1 and 2, HPV 16 and 18

6.png

  х 6 (six tubes per one sample)